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Figure 5


Fig. 5. Gata2 to Gata1 switch in occupancy at Eklf cis-regulatory regions during G1E-ER-Gata1 and EB differentiation. Quantitative ChIP analysis of Gata2 and Gata1 binding to cis-regulatory regions of the Eklf or Gata1 locus as indicated. Occupancy values recorded at a non-conserved GATA motif in the Eklf 3'UTR serve as baseline signal. (A) G1E-ER-Gata1 cells before (white) and after (black) 14 hours of estradiol treatment. (B) Differentiating EBs before (day 4, white) or after (day 6, black) erythroid commitment. Normal rabbit immunoglobulin (IgG) ChIP serves as negative control. Arithmetic mean of three independent experiments ±s.d. is shown.