Fig. 5. Gcm1/Synb/Cebpa-expressing cells are not required to induce or maintain Syna or Hand1 expression. (Left) In situ hybridizations for Hand1, Syna, Gcm1, Cebpa and Synb were performed on E8.5 and E9.5 wild-type and Gcm1^-/- mouse placentas. (Right) Northern blot analysis of Hand1, Ctsq, Syna, Gcm1, Cebpa and Synb expression in differentiating TS cultures following the removal of human recombinant FGF4, heparin and embryonic fibroblast-conditioned media. 18s rRNA was used as a loading control.