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Figure 6


Fig. 6. Gli3 is required to restrict Fgf expression to the isthmus. (A-X) Wnt1, Fgf8, Fgf17 and Spry1 RNA expression in mouse embryos at E10.5 and E12.5. Posterior mes, Is and r1 are shown (see Fig. 5K). Black arrowheads indicate normal expression, red arrowheads ectopic gene expression. (A-F) The Wnt1 expression domain is unaltered in Gli3 mutants. Fgf8 (G-I), Fgf17 (M-O), and Spry1 (S-U) domains are expanded into medial, but not lateral, r1 in E10.5 Gli3-/- mutants. In E12.5 En1-Gli3 cko mutants, ectopic expression of Fgf8 (J,K), Fgf17 (P,Q), and Spry1 (V,W) is restricted to the most posterior region of medial r1, where Wnt1 (D,E) is normally expressed. (L,R,X) Fgf8, Fgf17 and Spry1 expression is normal in Nes-Gli3 cko mutants. Scale bars: 200 µm.