Fig. 7. Partial rescue of the Gli3^-/- mutant phenotype in Gli3^-/-;Fgf8^+/- mutants. (A) H+E staining on Gli3^-/-;Fgf8^+/- mutant sagittal sections. The morphology of the Cb and Is, but not of the tectum (Sc and Ic), appears to be partially rescued in Gli3^-/-;Fgf8^+/- mutants. (B-D) En1 and Otx2 RNA expression and immunohistochemistry for neurogranin (D, red outline) show that the Ic is not properly established in Gli3^-/-;Fgf8^+/- mutants. (E,F) Math1 RNA expression (E) and DAPI staining (F, blue) show that the EGL expands from the posterior Cb to the Is (arrowheads), comparable to WT. (F,G) Immunohistochemistry for calbindin and ROR (green) show a relatively normal PCL, but a significant number of PCs are located in clusters in the deeper Cb and Is (G, outlined). (H) Pax2-positive cells (green) are found in the Is, but are excluded from PC clusters (arrowhead). Scale bars: 200 µm.