Fig. 5. CheWnt3 drives the development of oral fate. (A) Confocal images of early gastrula stage Clytia embryos stained with phalloidin (green) and To-Pro3 (red), showing characteristic phenotypes obtained following Wnt3 RNA injection into eggs before fertilisation. Presumptive endoderm cell ingression took place over a broad area of the embryo, with a graded distribution of CheBra apparent. (B) Equivalent observation at the 1-day planula stage, showing the lack of a distinguishable morphological oral-aboral axis. (C,D) Representative in situ hybridisation images of CheBra and FoxQ2A expression at early blastula stage (15 hpf). In embryos injected with CheWnt3 RNA, CheBra expression has expanded to the entire body with a residual graded distribution, and FoxQ2A expression has been lost. Scale bars: 40 µm.