Fig. 6. Restoration of oral poles in Wnt3-MO embryos by ectopic CheWnt3 expression. (A-C) Confocal images of early gastrula Clytia embryos stained with phalloidin (green) and ToPro3 (red). (B) Uninjected; (A,C) Injected with Wnt3-MO prior to fertilisation; for C, Wnt3 RNA was further injected into one blastomere at the 8-cell stage (n=15). In C, higher-magnification images of the region derived from the RNA-injected cell oral end, labelled by co-injected fluorescent dextran (blue), are shown beneath. Asterisks indicate the involution of cells neighbouring those containing Wnt3 RNA. (D) Representative in situ hybridisation image of CheBra (oral) and CheFoxQ2a (aboral) expression in early gastrulae (15 hpf) derived from Wnt3-MO-injected eggs (left). Wnt3 RNA was injected into a blastomere at the 4-cell stage after Wnt3-MO injection (right). Scale bars: 40 µm.