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Figure 1


Fig. 1. Conditional knock-in of KrasG12D in granulosa cells. (A-F) In vivo recombination of the R26R locus in ovaries by the Cyp19-Cre transgene. (A) ROSA26, (B) ROSA26;Cyp19-Cre and (C) ROSA26;Cyp19-Cre, 48 hours after eCG treatment. Images are of ovaries from 23-day-old mice showing β-gal staining (blue). (D) Day10 and (E) Day23 without eCG, and (F) Day23 with eCG treatment. Hematoxylin and Eosin staining of paraffin sections after β-gal staining showing the expression of β-gal in the ovaries of the ROSA26;Cyp19-Cre mouse. F, follicle; GC, granulosa cell. (G) RT-PCR detection of KrasG12D and total Kras mRNAs in LSL-KrasG12D;Amhr2-Cre and LSL-L-rasG12D;Cyp19-Cre mouse ovaries. (H) Immunofluorescence of KRAS in the ovary of a 6-week-old cycling wild-type mouse.