Fig. 2. Abnormal cortical plate development in C3G^gt/gt mutant mouse embryos. (A-I) Wild-type (A,C,F,H) and C3G^gt/gt mutant (B,D,E,G,I) developing cerebral cortex, at E13.5 and E14.5 as indicated, stained for the neuronal marker Map2 (brown in A,B; green in F-I) and/or laminin (red in C-I) and counterstained with bisbenzimide (blue in C-I). C3G^gt/gt mutants show disorganisation of the cortical plate and invasion of the pericerebral space by neurons (arrow in B). E13.5 developing cortex shows continuity of laminin staining along the basement membrane (asterisk) in the wild type (C) and the disorganisation (D) and discontinuity (arrows in E) of laminin staining in the E13.5 C3G^gt/gt mutant, as well as nuclei (blue) of cells protruding into the basement membrane gaps (arrow in E). The cells protruding into the basement membrane gaps are positive for the neuronal marker Map2 (arrows). Labels as in Fig. 1. Scale bar: 29 µm in A,B; 35 µm in C-I.