Fig. 4. Abnormal distribution of the basement membrane and of RC2-positive radial glial cells in the developing cortex of the C3G^gt/gt mutant. Epifluorescence (A,B,G,H) and confocal z-stack reconstructions (C-F) of frozen sections stained for laminin (red) and RC2 (green). Wild-type (A,C,E,G) and C3G^gt/gt mutant (B,D,F,H) mouse developing cerebral cortex at E12.5 to E14.5 as indicated. Wild-type radial glial processes (A,C,E,G, green, arrowheads in E) overlap (yellow, arrows in E) with basement membrane laminin (red). C3G^gt/gt mutant radial glial processes are entangled (B,D,F,H, long arrows in F) and show little overlap with laminin (short arrow in F). Asterisk, blood vessel; PS, pial surface; other labels as in Fig. 1. Scale bar: 25 µm in A,B; 249 µm in C; 242 µm in D; 137 µm in E; 141 µm in F; 11 µm in G,H.