Fig. 5. Abnormal distribution of reelin-expressing marginal zone cells in the developing cortex of the C3G^gt/gt mutant. (A,B) Confocal images of frozen sections stained for reelin (green) and counterstained with bisbenzimide (blue). (C,D) Differential interference contrast images of paraffin sections stained for calretinin (brown). (E-J) Epifluorescence images of frozen sections stained for reelin (green), laminin (red) and counterstained with bisbenzimide (blue). Wild-type (A,C,E,G,I) and C3G^gt/gt mutant (B,D,F,H,J) mouse developing cerebral cortex at E12.5 to E14.5 as indicated. Note that cells positive for reelin (A,B) and calretinin (C,D) are evenly distributed in the wild-type (arrowheads in A,C), but not in the C3G^gt/gt mutant (arrowheads in B,D) developing cortex. Also note that the gaps in reelin-positive cells coincide with gaps in laminin distribution (F,H,J, arrows). Scale bar: 25 µm in A-J.