Fig. 1. The temporal pattern of Phox2b expression in the embryonic pancreas and stomach. Phox2b mRNA levels were determined by real-time RT-PCR (TaqMan) of RNA isolated from the pancreas (A) and stomach (B) of mouse embryos from E10.5 to E15.5. All values are expressed relative to the level in pancreatic RNA at E10.5 and represent the mean value of three independent experiments performed in triplicate ±s.e.m. In A, mRNA values that are below or at the limit of detection (E10.5, E14.5 and E15.5) are displayed at the value for the limit of detection.