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Figure 2


Fig. 2. Premature and expanded placode development in β-catenin mutant embryos. (A-D) Premature placode development at E12.5 indicated by Hematoxylin and Eosin (H&E) staining (A,B, arrows) and Shh mRNA expression (C,D; purple brown) in KRT14-Cre Ctnnb1(Ex3)fl/+ (Act β-catenin) embryos compared with controls. (E-H) Placodes are enlarged in the mutant at E14.5 (E,F) and immunofluorescence for PGP9.5 reveals precocious development of enlarged nerve fibers (G,H, arrows). (I-P) Whole-mount in situ hybridization for Wnt10b (I-N) or Edar (O,P) reveals accelerated formation of enlarged placodes in the mutants. (Q-T) Whole-mount X-gal staining of control {kappa}Gal (Q,S) and KRT14-Cre Ctnnb1(Ex3)fl/+ {kappa}Gal (R,T) embryos reveals an irregular pattern of NF{kappa}B activity (arrows) in the mutants. Scale bars: bar in B applies to A,B; in D, to C,D; and in H, to E-H.