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Figure 1


Fig. 1. Insertional mutagenesis of Jaws causes chondrodysplasia. (A) Widespread β-galactosidase staining from the gene trap reporter in Jaws+/- E12.5 forelimb (left) and E15.5 digits (right). (B) Gross morphology (top) and cleared skeletal preparations (bottom) of wild-type (WT) and Jaws-/- embryos (E18.5). (C) Skeletal preparations from wild-type (top or left) and Jaws-/- (bottom or right) embryos, showing severe malformation of endochondral elements. (D) Alcian Blue/von Kossa staining of humerus sections (E17.5). The arrow indicates ectopic chondrocytes encroaching upon the primary ossification center. Higher-magnification views (top) show hypocellularity and ECM discontinuity in the Jaws-/- growth plate.