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Figure 3


Fig. 3. MTs function in initiating amnioserosa cell elongation. DE-Cadherin marks cell outlines. (A) Embryo stage/region measured. Transverse furrows below surface AS. PMGI, posterior midgut invagination. (B,E,G) 30-minute treatment with carrier. (C,F,H) 30-minute treatment with colchicine. (B,C) Stage 7-8 [transverse furrow cells columnar (not shown)]. Initial AS cell elongation occurs with carrier (B) but not with colchicine (C). (D) Cell elongation quantification. (E,F) Stage 8-9. Elongated surface cells with carrier (E), but not with colchicine (F). Elongated cells in transverse furrows in both (bracketed). (G,H) Stage 9-10. The full AS cell length is surface-exposed with carrier (G). Elongated cells show less surface exposure with colchicine (H, arrows). Scale bar: 5 µm.