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Figure 4


Fig. 4. Actin inhibits apical MT protrusion. (A-F) Stage 7-8. DE-Cadherin marks cell outlines. (A) Carrier. (B) Cytochalasin D. AS apical cell protrusions (bracketed, arrows). (C,D) AS cells in A,B are magnified. (E) Colchicine alone. (F) Simultaneous colchicine and cytochalasin D. (G,H) Stage 7-8 AS cells. DE-Cad (green). Dlg (red). (G) Carrier. (H) Cytochalasin D. (I-L) Stage 10 germband. (I-K) DE-Cad. (I) Carrier. Normal DE-Cad (bracketed). Normal contractile region (arrow). (J) Cytochalasin D. Clustered DE-Cad-positive protrusions (bracketed). (K) Simultaneous colchicine and cytochalasin D. Protrusions are suppressed (bracketed). (L) Cytochalasin D. MTs (red) in DE-Cad (green)-positive protrusions (arrow). Lateral MTs embryo surface-exposed (outlined). White/black scale bars 5 µm; gray bars, 25 µm.