Fig. 5. Amnioserosa extension through rapid cell elongation without intercalation. (A) Three-dimensional projections. DE-Cad-GFP. AS cell elongation. Four marked neighboring cells retain contacts. (B) Initial AS cell elongation rates. Blue lines indicate average elongation rate for five cells, DE-Cad-GFP embryos. Black lines indicate average elongation rate for five cells, Moesin ABD-GFP embryos. Red line indicates the all embryo average. (C) DE-Cadherin-GFP level quantification through confocal stacks. Background-corrected AP and DV contact values normalized to DV contact value for each cell. Five cell averages per embryo are shown (four embryos, different colors). Squares, DV contacts; circles, AP contacts (arrows). (D) Three-dimensional projections. DV contacts (white arrows), DE-Cad-GFP looping (yellow arrows). Scale bars: 5 µm.