Fig. 2. Phenotypic analyses of hsd-1. (A) Brood sizes of N2, hsd-1, ncr-1 and ncr-2 single mutants, as well as hsd-1; ncr-1 and ncr-2; ncr-1 double mutants. The number of parent worms (n) is indicated above each column. Error bars: standard deviation (s.d.); asterisks indicate significantly reduced brood sizes compared with N2 (P<0.01) by Student's t-test. (B) Responses of different mutant/transgenic strains to two different concentrations of dauer pheromone extract: 4 µl/plate (light grey) and 8 µl/plate (dark grey). Total numbers scored are above the corresponding columns with the error bars representing the s.d. based on at least three replicates. Number in parentheses indicate average population density (worms/plate) ±s.d.