Fig. 6. ns157 alters VAB-3, a Pax6/7-related protein. (A) vab-3(ns157) rescue studies. Strains scored as in Fig. 4. VL/R and DL/R, ventral left/right and dorsal left/right CEPsh glia, respectively. n, number of animals. F14F3, vab-3 cosmid. A representative line is shown for each construct. The vab-3 genomic locus construct contains 24 kb surrounding and including the gene. (B) C. elegans vab-3 gene structure. Boxes, exons; -shaped lines, introns. Those exons marked with asterisks are specific for isoforms B and C. Paired domain and homeodomain in blue. bx23 deletes isoform B exon 1. (C) Paired domain and homeodomain alignment of VAB-3 and indicated proteins. Pink, identical in all; blue, identical in all but one; gray, present in three of the proteins. (D) Mosaic analysis of vab-3 control of hlh-17::expression. +/-, transgene/hlh-17::GFP expression present/absent; n, number of animals. (E-H) Induction of hlh-17::GFP reporter expression by overexpression of vab-3 isoform A using a heat-inducible promoter. Merged DIC/fluorescence (E,G) and fluorescence (F,H) images of embryo without heat shock (E,F) and 90 minutes after heat shock (G,H).