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Figure 1


Fig. 1. GFP fluorescence in CAG-GFP transgenic chickens. (A) The provirus is flanked by self-inactivating LTRs ({Delta}LTR), and contains the virus packaging site ({Psi}) and a neomycin resistance open reading frame 5' to the CAG-eGFP transgene. (B-E) GFP fluorescence in transgenic embryos at (B) new laid egg stage, (C) stage 4 HH, (D) stage 15 HH and (E) 5 days (transgenic on left). (F,G) Transverse sections of (F) stage 15 HH GFP+ and control embryos, and (G) day 5 GFP+ and control embryos. (H) Stage 13 HH embryos hybridised for GFP (transgenic on left). (I) Flow cytometric analysis of GFP fluorescence (stage 11 HH embryos). Black line, non-transgenic embryo; blue and green lines, transgenic lines 158 and 205, respectively. Scale bars: 0.5 mm.