Fig. 5. Wg acts upstream of Ss and independently of it in transforming maxillary
identity to antennal. (A1-A4)
Light-microscope images of adult maxillary palps. (A1) Wild-type
maxillary palps with their bordering row of bristles. (A2)
ss- null homozygote shows strongly reduced maxillary
palps. (A3) wggof; ss-
double mutants (shifted to 18°C at 5 days AEL) generally have an enlarged
structure, unlike either wggof neo-antennae or
ss- stubs. These structures are positioned higher on the
head, like neo-antennae (as seen in Fig.
3B). (A4) Same genotype as in A3, but with
the maxillary palp replaced by distal leg with its distinctive claws.
(B-E) Dan (green) and Dfd (red) expression in L3 E-A of (B) a wild-type
antennal disc, (C) a wggof-induced transformation
(ptc-GAL4>UAS-wgts shifted to 18°C at 5 days AEL),
(D) a ssgof-induced transformation
(ptc-Gal4>UAS-ss), (E) a wggof; ss-
disc (shifted to 18°C at 5 days AEL) where Dfd (red) is excluded from the
maxillary territory (arrowhead). (F,F') In
ssgof discs harbouring large pygo-
clones (no GFP), Dan (red) is expressed in a narrow band of maxillary cells
(F, arrowhead) and Dfd expression (red) is maintained (F', arrowhead).
(G) Schematic summary of results. Antennal discs are oriented with
posterior towards the right and dorsal towards the bottom; a and mx indicate
antenna and maxillary primordia, respectively.