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Figure 3


Fig. 3. Mutant sensory axons fail to respond to ventral spinal cord. (A-C) Co-cultures of wild-type E13.5 ventral spinal cord (VSC) with DRG from E13.5 wild-type (A), L1-null (B) or TAG-1-null (C) embryos in collagen gels. (D) Quantitation of axon growth: DRG were divided into quadrants and axons in each counted to give proximal:distal (P/D) ratio. (E) Graph showing the P/D ratios in wild type (WT; mean=0.269, s.e.m.=0.06, n=27), L1 null (mean=0.713, s.e.m.=0.043, n=15), TAG null (mean=1.03, s.e.m.=0.08, n=15), wild type plus polyclonal anti-TAG-1 (mean=0.67, s.e.m.=0.02, n=7) and wild type plus PI-PLC (mean=0.72, s.e.m.=0.06, n=7). ***P<0.001, **P<0.01, *P<0.05; unpaired t-test. Similar results were obtained counting axon length (not shown). Scale bars: 100 µm.