Fig. 5. TAG-1 does not bind neuropilin 1, plexin A4 or Sema3A. Cos7 cells transfected with full-length L1 (A,D), TAG-1 (B,E,I,J), NRP1 (C,F) or plexin A4 (K,L) and probed with L1-Fc (A-C), TAG-1-Fc protein (D-F,K,L) or Sema3A-Flag (I,J). Quantification of the percentage of cells binding the probe is presented in G (L1-Fc), H (TAG-1-Fc) and M (Sema3A-Flag). ^***P<0.001, ^**P<0.01; unpaired t-test. The transfection efficiency (as assessed by immunolabelling; shown for anti-TAG-1 (I) and anti-Myc (detects plexin A4-myc; K) was similar in each case.