Fig. 2. dmd-3 and mab-3 reporters are sex-specifically expressed in the tail tip hypodermis. (A) dmd-3::YFP (fsIs2) expression in an L4 male (i) demonstrating expression in the hindgut, B lineage, tail tip (hyp8-11) and hyp13. The future position of the dmd-3-expressing RnA neurons is marked with a broken white line. An L4 hermaphrodite carrying dmd-3::YFP (ii) demonstrates the male-specificity of expression. In late-L3 (iii) and L4 males, dmd-3::YFP is expressed in the linker cell of the developing male gonad. (B) Schematic diagrams of the progression of tail tip morphogenesis. Circles indicate tail tip hypodermal cells; green shading reflects the intensity of dmd-3 expression. Solid lines between hypodermal cells indicate intact boundaries and broken lines indicate cell fusion events. The thick black line represents the L4 cuticle, whereas the thick gray line indicates the newly formed adult cuticle. See Materials and methods for a description of L4 staging criteria. (C,D) Confocal microscopy images of L4 males (i-vi) and hermaphrodites (vii) carrying dmd-3::YFP (fsIs2) (C) or MAB-3::GFP (fsIs10) (D). Numbers indicate tail tip hypodermal cells. hg and RnA indicate the hindgut and ray RnA neurons, respectively. The larval cuticle is outlined with dashed lines; dotted lines indicate the developing adult cuticle.