Fig. 5. EFF-1 is regulated by dmd-3 and mab-3. (A) AJM-1::GFP (syIs78)/DIC and DIC images of wild-type and eff-1(ok1021) L4 (i,ii) and adult (iii,iv) males. The broken green arrowhead indicates cell fusion; the solid green arrowhead marks intact cell boundaries. The white arrows indicate tail tip retraction. (B) Tail tip expression of an EFF-1::GFP translational reporter (fsEx135) in male (i-v) and hermaphrodite (vi) larvae. (C) Expression of EFF-1::GFP in wild type (i), mab-3 (ii), dmd-3 (iii,iv) and dmd-3; mab-3 (v) mid-L4 males. The examples in iii and iv represent the range of EFF-1::GFP expression levels seen in dmd-3 mutants. (D) Categorization of EFF-1::GFP fluorescence intensity in wildtype (n=39), mab-3 (n=24), dmd-3 (n=61) and mab-3; dmd-3 (n=33) mid-L4 males and wild-type mid-L4 hermaphrodites (n=31) based on confocal images. (E) Expression of EFF-1::GFP::outron::mCherry in a wild-type mid-L4 male (i-iii) and hermaphrodite (iv-vi). GFP (i,iv), mCherry (ii,v) and GFP/mCherry (iii,vi) overlays are shown.