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Figure 3


Fig. 3. Increased production of neurons in mib and DAPT-treated embryos. (A) Average numbers of Islet1+ cells in the epiphysis of mock-treated embryos, mib mutant and DAPT-treated embryos at 48 hours. (B-G) Dorsal view of epiphysis, showing expression of flh, ascl1a and ngn1 in mock or DAPT-treated embryos at 16 hours. Error bars represent the standard deviation. *P<0.05; ***P<0.0005 using a t-test. Scale bar: 10 µm. Anterior is upwards.