Fig. 6. Cell-cell and cell-matrix adhesion are affected in morphant cells. (A) Morphant dorsal axial cells at stage 13 exhibit a dramatic dose-dependent reduction in adhesion to recombinant extracellular domain of C-cadherin (C-Cad). (B) However, cell-surface levels of C-Cad are unchanged in morphant cells, as similar levels of control and morphant cell C-Cad is available on the cell surface for trypsin digestion. (C) Morphant cells also show a dramatic reduction in adhesion to fibronectin (FN). (D) Cell-surface levels of integrin 5 are not affected by myosin IIB depletion. (E) Failure of blastopore closure due to MHC-B depletion is not rescued by exogenous expression of C-Cad, consistent with the hypothesis that C-Cad levels are not limiting in morphant embryos. (F) Adhesion to FN in morphant cells is rescued by co-expression of exogenous human MHC-B.