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Figure 5


Fig. 5. Expression of the PTB domain, but not the PRR domain, affects non-skeletogenic mesoderm specification. (A-C) Pigment cells. (D-F) Pluteus larvae stained with SMC2. (A,D) Control embryos. (B,E) Injection of ptb domain mRNA (2 pg/pl) increases the formation of pigment cells and blastocoelar cells (arrowheads indicate large aggregations of blastocoelar cells). (C,F) Pigment cell and blastocoelar cell specification is unaffected by expression of Myc-PRR domain (3-6 pg/pl) (image shown) or untagged LvPRR domain (3-6 pg/pl) (data not shown). (G) Western blot showing expression of Myc-PRR domain protein in early gastrula-stage embryos. The protein is the predicted size of 52 kDa. (H) Schematics showing the LvNumb constructs used for experiments. The PTB domain, full-length LvNumb, the PRR domain and EH domains are indicated.