Development -- Dubois et al. 135 (14): 2455 Figure IG1

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Fig. 1. Sox2-Cre mediated epiblast-specific deletion of c-myc reveals viable embryos up to E11.5. (A) Schematic diagram showing the conditional c-myc^flox allele (Trumpp et al., 2001). Exons are indicated as boxes 1-3. The open reading frame (ATG to Stop; yellow segments) is flanked by loxP sites (black triangles). Restriction sites and probe (blue ovals) used for Southern blot analysis are indicated. (B) Southern blot showing recombination at the c-myc locus in c-myc^flox^/^ORF^rec (cont) and Sox2Cre;c-myc^flox^/^flox (exp) embryo and c-myc^flox^/^flox (cont) and Sox2Cre;c-myc^flox^/^flox (exp) placenta at E10.5. (C) Real-time RT-PCR analysis of c-myc, N-myc and L-myc expression in Sox2Cre;c-myc^flox^/^flox embryos at E10.5. Expression levels in mutants are normalized to that in controls, which was set to 1. (D) Phenotypes of control (left) and Sox2Cre;c-myc^flox^/^flox embryos (right) at (i) E9.5, (ii) E10.5 and YS, and (iii) E11.5.