Fig. 3. Tie2-Cre-mediated c-myc deletion did not affect allantoic or placental vasculature but diminished hemogenic endothelial cells in the aorta. (A,B) Allantoic explants stained for CD31 show no obvious difference between the control and mutant. (C,D) Placental vasculature at 11.5 dpc revealed by Tie2-lacZ reporter assay shows no obvious differences between the control and mutant. Arrowheads indicate the blood vessels. (E,F) H&E stained cross-section of dorsal aorta shows hemogenic endothelial cells (arrows) in the control but not in the mutant. Scale bars: 50 µm.