Fig. 7. Expression of proangiogenic factors in Vav-iCre;c-myc^flox/- and Tie2-Cre;c-myc^flox/- mutant embryos. (A) mRNA levels of proangiogenic factors in Vav-iCre;c-myc^flox/- at 11.25 dpc. Real-time PCR was performed using total RNA from five mutants and 15 control embryos (Vav-iCre;c-myc^flox/+, c-myc^+/- or Vav-iCre;c-myc^+/-). The statistical significance of the differences in means between controls (relative expression=1) and mutants are listed by their P values. (B) Increase in VEGF protein levels by ELISA in control and Tie2-Cre;c-myc^flox/- embryos (n=5). VEGF levels in the Tie2-Cre mutant embryos were significantly elevated (^*P<0.01).