Fig. 3. OE embryos display elevated Cdx protein concentrations and normal location of mRNA expression. (A) Western blots showing time-course of Cdx1, Cdx2 and Cdx4 protein levels in the primitive streak/tailbud region of normal embryos. Replicate tissue samples are examined for each protein. Cdx1 bands lie just below a 37 kDa marker protein (not shown); Cdx2 and Cdx4 bands lie just above. (B) Western blot showing time-course of Cdx2 protein in OE2 line 1 embryos. (C) Western blots comparing Cdx protein concentrations in tailbuds of stage-matched transgenic versus non-transgenic (NT) littermates sired by Cdx overexpresser (OE) mice. Results are shown for lines 1 and 2 of each OE type, and the Cdx2 blot also includes embryos sired by the peak-expresser stud. The fold-levels of overexpression for each OE line, assessed by densitometry, are indicated. (D) Expression of OE transgenes (line 1 for each) detected by in situ hybridization of the lacZ/SV40 probe to 8.5 to 8.7 day embryos.