Fig. 4. The C&E movements of mesoderm and ectoderm are not affected by Sdf1/Cxcr4a signaling during zebrafish gastrulation. (A-D) The position and shape of the prechordal plate (pcp), the anterior edge of the neural plate (np) and notochord (nt) at the three-somite stage. Embryos were stained for no tail (ntl; notochord marker), hatching gland gene 1 (hgg1; prechordal plate marker) and dlx3 (anterior edge of neuroectoderm) by in situ hybridization. Dorsal views, anterior to the top. No significant differences in the expression patterns of these three genes were found in either the cxcr4a or sdf1 knockdown embryos as compared with misMO1-injected embryos. White lines indicate the gap between the pcp and nt. (E-H) The body length of embryos at the three-somite stage, marked by the expression of myoD. Dorsal views, anterior to the top. The expression pattern of myoD in the cxcr4a or sdf1 knockdown embryos is indistinguishable from that in the misMO1-injected embryos.