Fig. 5. Cxcr4a signaling cell-autonomously regulates the movements of endodermal cells. (A-F) Confocal images of the endodermal cells in Tg(sox17:EGFP) transgenic zebrafish embryos at the three-somite stage. Dorsal views, anterior to the top. One marginal blastomere cell at the 128-cell stage has been transformed into an endodermal fate by the misexpression of cas mRNA. The injected cell was labeled with dextran-Alexa594. (A-C) The progeny of the injected cell intermingle with EGFP-expressing endodermal cells. (D-F) The migration of both cas mRNA and cxcr4aMO1 co-injected cells was significantly delayed compared with cells injected with cas mRNA only (arrowheads, E,F). (C,F) Note that the width from the midline to the lateral margin of the endodermal cell layer in the cas mRNA + cxcr4aMO1 co-injected embryos is broader than that of the cas mRNA-injected embryos because the movement of the endodermal cells is delayed (areas medial and lateral to the midline are indicated by the double-headed arrows).