Fig. 6. Bmp signaling and homeodomain gene expression in Hes1;Hes3;Hes5 cKO mice. (A-G') At E11.5, in Hes1;Hes3;Hes5 cKO mice, the expression domains of Bmp4, Lmx1a, Msx1, Msx2 and Wnt3a were reduced in size, compared with the control, although Foxg1 and Lhx2 expression (cortex) was not significantly affected. (H-M') At E12.5, in the control, the telencephalic midline region was clearly separated into the choroid plexus epithelium and the cortical hem, and Bmp4 and Lmx1a were expressed in both regions (H,I). In Hes1;Hes3;Hes5 cKO mice, the prospective choroid plexus region remained pseudostratified, and Bmp4 and Lmx1a expression domain was smaller in size (H',I'). Msx1 and Msx2 expression domains were also reduced in size (J',K', asterisks), whereas expression of Wnt3a (cortical hem) and Lhx5 (eminentia thalami) was not significantly affected (L',M'). Scale bars: 100 µm in A-G'; 200 µm in H-M'.