Fig. 1. The DSL ligand lag-2 is expressed in the three pairs of IL2 neurons during the dauer stage. (A) A daf-7(e1372) dauer larva expressing the lag-2::GFP transgene and the corresponding DIC image overlaid with the GFP expression to show the position of the cells expressing the transgene. White arrowheads indicate the described expression of lag-2::GFP in the distal tip cells (DTC). Scale bar: 25 µm. (B) The head region of dauers expressing lag-2::GFP (qIs56) induced by either pheromone, or in various Daf-c mutants as indicated in the panels. (C) 3D reconstruction of a confocal stack of images of the IL2 neurons in dauer. White lines outline the pharynx of the dauer animal. Scale bars: 10 µm. (D) Diagram of the IL2 neurons indicating their position and their characteristic morphology (adapted with permission from wormatlas.org). (E) Merge of dauer expressing lag-2::GFP (green) in the IL2 neurons that were stained with the lipophilic dye DiI (red). As lag-2::GFP is expressed in the entire cell, whereas DiI only stains the membrane, colocalisation (yellow) is only observed at the membrane, giving a halo-like appearance. In all images, arrowheads indicate the IL2 neurons.