Fig. 3. The forkhead transcription factor UNC-130 is required for appropriate repression of lag-2 expression during reproductive development. (A) Expression in the head region of the various forkhead transcription factors predicted from the C. elegans genome database during the dauer stage in daf-7(e1372) mutants (Hope et al., 2004). (B) Confocal images depicting the expression of the UNC-130::RFP translational fusion protein in the daf-2(e1370); qIs56 (lag-2::GFP) background, and the merge of the two channels during the dauer stage. (C) The percentage of L3 larvae kept at sub-threshold temperature (20°C) that express lag-2::GFP in the IL2 neurons in the mutant background is indicated.