Fig. 4. GLP-1 is expressed in differentiated neurons in the head during dauer. (A) The glp-1p::GLP-1::YFP transgene is expressed in two head neurons during dauer in a daf-7(e1372) background. The cell bodies (indicated by arrow) are located in close proximity to the terminal bulb. The axon of these neurons (arrowheads) projects toward the anterior into the nerve ring, where they are within close proximity of the IL2 axons. Scale bar: 5 µm. (B,C) The same construct was injected in a rab-7(ok511)II/mIn1; daf-7(e1372) mutant, which disrupts the endocytic-mediated receptor recycling pathway. YFP-containing vesicles accumulate in the same neurons as in A. (C) Confocal micrograph depicting the accumulation of YFP vesicles along the length of the animal in neurons within the ventral cord. Scale bar: 20 µm. rol-6 was used as co-transformation marker, which accounts for the abnormal morphology of the ventral cord neuronal processes. Anterior is to the left in C.