Fig. 2. Cre expression in the adult adrenal gland. (A) Southern blot analysis of isolated genomic DNA from wild-type, Sf1/Cre^low, and Sf1/Cre^high mice. (B) LacZ activity staining of adrenal glands from 6-week-old male Z/AP, Sf1/Cre^low x Z/AP and Sf1/Cre^high x Z/AP, as described in the Materials and methods. Scale bars: 100 µm. (C) Quantitative-PCR analysis of hAP expression in adrenal glands from 6-week-old male wild-type, Z/AP, Sf1/Cre^low x Z/AP and Sf1/Cre^high x Z/AP mice.