Fig. 3. Comparison of wild type and Sf1/Cre^high-mediated β-catenin KO embryos at different developmental stages. Embryos were harvested from timed-pregnant dams at the indicated stages and sagittal sections were processed and analyzed as described in the Materials and methods. (A) Immunohistochemical analysis of β-catenin expression. (B) Hematoxylin and Eosin (H&E) staining of sections. (C) Immunohistochemical analysis of SF1 expression using antiserum B against Sf1 to identify adrenocortical progenitors. The offset shows a section from an E16.5 embryo stained with an antiserum against tyrosine hydroxylase, which detects chromaffin cell precursors. DA, dorsal aorta; G, gonad. Scale bars: 100 µm.