Fig. 5. Loss of β-catenin mediated by the Sf1/Cre^high transgene results in a decreased number of adrenocortical cells at least partly due to decreased proliferation. BrdU incorporation into wild-type and Sf1/Cre^high-mediated KO mice was determined at the indicated stages as described in the Materials and methods. Mice with Sf1/Cre^high-mediated β-catenin KO appeared to have decreased BrdU staining in the region of the adrenal primordium. The graphs show quantitation of Sf1-positive cells (top) and Sf1-positive cells that were also positive for BrdU (bottom) in E12.5 and E13.5 embryos. The number of Sf1-positive cells was decreased significantly at both E12.5 (^*P<0.01 versus wild type) and E13.5 (^**P<0.01 versus wild type), whereas the number of doubly labeled cells was only decreased significantly at E13.5 (^**P<0.01 versus wild type). Scale bars: 50 µm.