Fig. 7. Progressive decline of adrenocortical volume in Sf1/Cre^low-mediated β-catenin KO mice. (A) Histological analysis of the adrenal glands from wild-type and Sf1/Cre^low-mediated β-catenin KO mice at different ages. Adrenal glands were isolated from mice of the indicated genotypes as described in Materials and methods, and processed for immunohistochemical detection of Sf1 (antiserum A), β-catenin and tyrosine hydroxylase (Th). TUNEL assay was conducted on the section as described in the Materials and methods. (B) H&E staining and Sf1 immunohistochemistry (antiserum A) of adrenal from wild type and Sf1/Cre^low-mediated β-catenin KO mice at 45 weeks of age. The black bar highlights the adrenal cortex. Scale bars: 100 µm. C, adrenal cortex; M, adrenal medulla.