Fig. 2. Ribosomal components display increased solubility in Padi6^-/- oocytes. (A) qRT-PCR analysis using 18S rRNA primers and western blot analysis using anti-S6 antibodies indicates that levels of ribosomal components are similar between Padi6^+/+ and Padi6^-/- oocytes. (B) In contrast with Padi6^+/+ oocytes, the majority of ribosomal protein S6 partitions in the supernatant of ruptured Padi6^-/- oocytes. Oocytes were ruptured in hypotonic buffer, serially centrifuged at 650 and 9000 g for 5 minutes, and the partitioning of ribosomal components into the supernatant (Sup) and pellet fractions was evaluated by immuno-slot-blot analysis using anti-S6 antibodies. (C) Transmission electron microscopic analysis indicates that although putative CPLs (arrow) are observed in the Padi6^+/+ 9000 g oocyte pellet, (D) lattices are not observed in the Padi6^-/- oocyte pellet. Arrowheads highlight ribosome-like particles associated with CPLs. Scale bar:100 nm. Values for the qRT-PCR are represented as the mean±s.e.m. from three independent experiments (P=0.407). The immunoblotting experiments were repeated three times and the electron microscopy experiments were repeated twice.