Fig. 3. PADI6 and ribosomal protein S6 colocalize in Triton X-100 extracted oocytes and loss of PADI6 alters S6 localization but not de novo protein synthesis. (A) PADI6 (P6) colocalizes with ribosomal protein S6 in Triton X-100 extracted GV stage Padi6^+/+ oocytes and S6 localization is altered in Padi6^-/- oocytes. PADI6 and S6 co-localization in Padi6^+/+ oocytes is highlighted in merged image (M). The degree of co-localization (oval) is shown in the scatter plot. S6 localization to the oocyte cortex (or lack thereof in the Padi6^-/- oocytes) is highlighted by the arrowheads. (B) Phalloidin staining of Triton X-100 extracted GV oocytes reveals that the cortical actin network is not disrupted in Padi6^-/- oocytes. (C) Wide-field immunofluorescence analysis shows that DNMT1 displays similar cortical localization in Padi6 ^+/+ and Padi6^-/- GV stage oocytes. (D) Fluorographic analysis of [³⁵S]methionine labeled proteins from Padi6^+/+ and Padi6^-/- GV stage oocytes. Arrow indicates a protein that, based on its molecular weight (72 kDa) and absence from Padi6^-/-oocytes, is probably PADI6. These experiments were repeated three times.