Fig. 5. Embryonic genome activation is defective in Padi6^-/- two-cell embryos. (A) Levels of RNA polymerase II (RNA Pol II), phosphorylated RNA Pol II (P-Ser2-CTD) and acetylated histone H4 (H4K5 acetyl) are significantly reduced in Padi6^-/- two-cell embryos. Representative nuclei shown were chosen based on their average signal intensity matching the respective mean of average intensities for Padi6^+/+ and Padi6^-/- embryo nuclei. Values represent the mean signal intensity±s.e.m. (B) BrUTP incorporation into the nucleus of Padi6^-/- two-cell embryos is significantly reduced. Representative nuclei are shown above bars in histogram. Values represent the mean BrUTP nuclear incorporation±s.e.m. from three independent experiments (P<0.05). (C) Fluorographic analysis of [³⁵S]methionine labeled and Triton X-100 extracted two-cell embryos indicates that synthesis of transcription requiring complex (TRC) proteins is reduced in Padi6^-/- embryos. These experiments were repeated three times.