Fig. 4. Early presynaptic dFMRP rescues dfmr1-null NMJ architectural phenotypes. (A) Representative images showing NMJs from Drosophila either vehicle- (GS+E) or experimentally treated (GS+RU486 at 0.25 mg/mL, R0.25) for 12 hours immediately post-hatching and then analyzed as third instar larvae (108 hours AEL), co-labeled for HRP and DLG. (B-E) Quantification of NMJ structure. Statistically significant rescue occurs for synaptic branch number (B), synaptic bouton number (C), presynaptic junctional area (D), and postsynaptic junction area (E). n=12-14 animals for each condition. Bars indicate mean±s.e.m. Dashed red lines highlight dfmr1-null conditions. ^*P<0.05, ^**P<0.01, ^***P<0.001.