Fig. 5. Acute late presynaptic dFMRP partially rescues dfmr1-null NMJ structure. (A) Depiction of time line employed for late larval stage intervention. Drosophila larvae were raised to 96 hours AEL, transferred to RU486-containing food for 12 hours, and then immediately processed. (B) Representative western blot of third instar larval CNS probed for dFMRP and -Tubulin. (C) Quantification of western blot dFMRP levels. Individual band intensities were normalized to -Tubulin and expressed as a percentage of the control. Bars indicate mean±s.e.m. ^**P<0.01, ^***P<0.001. (D) Representative NMJ images from animals treated during the late 12-hour time period, co-labeled for HRP and DLG. Acute dFMRP expression reduces the excess number of boutons observed in the dfmr1-null. (E) Quantification of synaptic bouton number. Late 12-hour treatment with RU486 to induce dFMRP effects partial rescue of NMJ structural alterations. n=10-12 animals for each condition. Bars indicate mean±s.e.m. Dashed red line highlights the dfmr1-null condition. ^**P<0.01.