Fig. 4. Crol regulates G1-S and G2-M cell cycle genes. (A) Adult
wings overexpressing UAS-crol with en-GAL4 and with the
following cell cycle mutants: (B) dmPO, (C)
cycEAR95, (D) stgAR2,
(E) cycB2, (F) E2F1. A tracing of
the PC from en-GAL4/+, UAS-crol/+ has been superimposed to show
relative modification of compartment size. (G-I) β-gal (red) of
120-hour wing discs for PL35/+;
en-GAL4,UAS-GFP/UAS-crol;UAS-p35/+. (J-L) β-gal
(red) on 96-hour wing discs for stg-lacZ 6.4/+;
Ptc-GAL4,UAS-GFP/UAS-crol; UAS-p35/+. (M) Control PCNA-GFP in
the 120-hour wing disc with the PC marked by En (red). (N)
en-GAL4/UAS-crol; UAS-p35/PCNA-GFP, merged with En antibody
in red (O).