Fig. 5. Crol inhibits Wg transcription. (A) crol overexpression wing phenotype alone and with (B) arm^YD35/+; (C) Axin^E77/+ and (D) Sgg¹/+. (E-G) crol mutant clones in the arm^YD35/+ background, positively marked with GFP and counterstained for DNA (red), compare with Fig. 2C,D. β-gal staining (red) for control en-GAL4, UAS-GFP/wg-lacZ (H-J) and en-GAL4, UAS-GFP/wg-lacZ; UAS-Crol/+ (K-M). (J,M) Merge with DAPI to show cells present in the apical region of the disc. Wg antibody (red) on control clones (N-P) and crol mutant clones generated in the Minute background (Q-S) are marked by the absence (arrows) of βgal (green). (T-Y) Arrows indicate crol clones adjacent to the ZNC (T-V) and the hinge (W-Y), and ectopic Wg in small clones are highlighted with asterisks (W-Y). (Z) Q-RT-PCR for wg mRNA in third instar larval imaginal tissues: Actin-GAL4/+ (1.0±0.21); Actin-GAL4/UAS-crol, (0.34±0.03). PCR was carried out in triplicate and normalized using GAPDH. The level of Wg mRNA is significantly reduced in Actin-GAL4/UAS-crol compared with Actin-GAL4/+ (P=0.0376).