Fig. 7. Localization of Shh protein in the developing dorsal pallium. Shh protein was localized in MZ and SVZ/VZ of the wild-type dorsal pallium at E13.5 (A); there are few Shh-positive cells in Shh-CKO mutants (A'). At E15.5, Shh immunoreactivity was also found additionally in IZ and CP (B). Note that MZ and IZ were more intensely stained than other zones at E15.5. In Shh-CKO mutants, the Shh-immunoreactivity was sustained in IZ and MZ (B'). N-cadherin was intensely expressed in IZ (D), and Shh protein (C) was colocalized with N-cadherin in IZ (E). Reelin and calretinin, CR cell markers, were detected in some of Shh-positive cells in MZ at E13.5 (F,G, arrows). Shh protein was co-expressed in GABAergic interneurons in MZ at E15.5 (H, arrows). Sections were counterstained with DAPI (dark blue nuclei). The antibodies and colors used in each panel are as indicated. Scale bar: 100 µm in A-E; 50 µm in F,G.