Fig. 1. CTCF depletion in oocytes and embryos. (A) Quantification of nuclear CTCF immunofluorescence staining in growing oocytes. Ntg and Tg indicate oocytes derived from 10-day-old Ntg and Tg littermates, respectively. Lines 1, 12 and 21 are shown. ^*P<0.0001; n=number of oocytes. (B) Immunofluorescence images of CTCF-stained oocytes and embryos. One confocal section of an egg or embryo is shown per panel, with red indicating CTCF-stained nuclei. Line 1 is shown. Scale bar: 40 µm. (C) Schematic of CTCF depletion in oocytes and embryos. Ntg females were mated to Tg males and 50% of female progeny inherited the transgene. All oocytes from Tg females were CTCF depleted. CTCF-replete oocytes and embryos are depicted in brown, whereas CTCF-depleted oocytes and embryos are depicted in white. Pink and blue circles represent maternal and paternal pronuclei, respectively. GV, germinal vesicle; hr, hours post-hCG.